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PML RARA Quantitative
Real-Time PCR Kit

  • SKU

    • 100 Rxns : NPRQN22122

    • 50 Rxns : NPRQN22122_01

    • 20 Rxns : NPRQN22122_02

PML-RARA Quantitative Real-Time PCR kit is an in-vitro multiplex, one-step RT-PCR assay for the specific differentiation and quantification of PML-RARA transcripts - bcr1 (long)/bcr2 (variant) and bcr3 (short) in the RNA extracted from peripheral blood or bone marrow aspirate samples of human, along with the Human internal control ABL1 in a single-tube assay to aid in diagnosing the presence of Acute Promyelocytic leukemia.

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Features
  • Single-Tube, One-Step, Multiplex, TaqMan probe-based Qualitative Assay: Detects 3 Target genes + 1 human internal control 

  • The limit of Detection is 10 copies/μL

  • The kit supports reverse transcription. No additional cDNA synthesis required

  • PCR Run Time

  • Single-Tube, One-step, Multiplex, TaqMan probe-based Quantitative Assay: Detects and differentiates 3 Target genes + 1 human internal control

  • Limit of Detection:

    • BCR1/BCR2 is 20 copies /μL

    • BCR3 is 8 copies /μL

    • ABL1 is 2.7 copies/μL

  • The kit supports reverse transcription. No additional cDNA synthesis required

  • PCR Run Time is within 75 Minutes

  • Human Internal Control - ABL1

  • Open Platform Kit compatible with BIORAD-CFX96, THERMO -QS5, QIAGEN-ROTOR - GENE Q and other instruments which supports FAM (495 nm - 520 nm), Cy5 (649 nm - 670 nm) and Texas Red (589 nm - 615 nm).

  • Traceability/Standards:

    • Qiagen’s Ipsogen PML-RARA bcr1 standards (Id:672191), bcr2 standards (Id:672291) and bcr3 standards (Id:672391)

Storage & Precautions

  • Recommended Storage: -20°C

  • For Professional Use only.

Clinical Relevance

Promyelocytic leukemia or retinoic acid receptor alpha or PML-RARA is an abnormal fusion gene sequence formed when pieces of chromosome 15 and chromosome 17 break off and switch places (translocate). 

 

The PML gene region in chromosome 15 then fuses with the RARA gene region in chromosome 17 making reciprocal translocation, and this particular one is commonly expressed as t(15;17). 

 

This test detects PML-RARA fusion in the blood or bone marrow to determine if an individual has acute promyelocytic leukemia (APL), a subtype of acute myeloid leukemia (AML). Detection of PML RARA fusion is considered to be a diagnostic hallmark of Acute Promyelocytic Leukemia (APL).

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