PML-RARA Qualitative
Real-Time PCR Kit
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SKU
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100 Rxns : NPRQ22073
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50 Rxns : NPRQ22073_01
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20 Rxns : NPRQ22073_02
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PML-RARA Qualitative Real-Time PCR Kit is an in-vitro multiplex, one-step RT-PCR assay for the qualitative detection of PML-RARA transcripts (bcr1, bcr2 & bcr3) in the RNA extracted from peripheral blood or bone marrow aspirate samples of human, along with Human internal control ABL1 in a single-tube assay.
Features
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Single-Tube, One-Step, Multiplex, TaqMan probe-based Qualitative Assay: Detects 3 Target genes + 1 human internal control
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The limit of Detection is 10 copies/μL
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The kit supports reverse transcription. No additional cDNA synthesis required
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PCR Run Time is within 75 Minutes
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Human Internal Control - ABL1
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Open Platform Kit
Storage & Precautions
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Recommended Storage: -20°C
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For Professional Use only.
Clinical Relevance
Promyelocytic leukemia or retinoic acid receptor alpha or PML-RARA is an abnormal fusion gene sequence formed when pieces of chromosome 15 and chromosome 17 break off and switch places (translocate).
The PML gene region in chromosome 15 then fuses with the RARA gene region in chromosome 17 making reciprocal translocation, and this particular one is commonly expressed as t(15;17).
This test detects PML-RARA fusion in the blood or bone marrow to determine if an individual has acute promyelocytic leukemia (APL), a subtype of acute myeloid leukemia (AML). Detection of PML RARA fusion is considered to be a diagnostic hallmark of Acute Promyelocytic Leukemia (APL).