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BRAF V600
mutation Detection Kit

  • SKU

    • 100 Rxns : NBRQ22076

    • 50 Rxns : NBRQ22076_01

    • 20 Rxns : NBRQ22076_02

BRAF V600 Mutation Detection Kit is an in-vitro multiplex, Real-Time PCR assay for the qualitative detection of V600 Mutations in the BRAF gene in human genomic DNA extracted from fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tumor tissue. The kit is for in-vitro diagnostic use and is intended to be used by trained professionals in a laboratory environment. The Kit differentiates the V600E mutation from the other V600 mutations like K/D/Ecomplex/M/R/G.

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Features
  • Single-Tube, Multiplex, TaqMan probe-based Qualitative Assay along with BRAF Wild 

  • RT-PCR Run Time is within 70 Minutes

  • Open Platform Kit compatible with commercially available RT-PCR systems

  • Traceability: HD238 BRAF V600E gDNA Reference Standard, 50%

  • Sample Compatability: fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tumor tissue.

  • Selective amplification of mutant-specific sequences in a mixture of wild-type and mutant DNA.

Storage & Precautions

  • Recommended Storage: -20°C

  • For Professional Use only.

  • Read user manual

Clinical Relevance

BRAF mutations have been identified at a high frequency in specific cancers, including approximately 50 to 60% of melanoma. The cancers associated with the BRAF gene mutation are not specific to one part of the body or a certain cell type. These cancers include Melanoma, Hairy Cell Leukemia, non-Hodgkin lymphoma, Thyroid Cancer, Ovarian Cancer, Lung adenocarcinoma, Colorectal cancer, and Certain brain cancers including glioblastoma. 

 

Approximately 90% of all identified BRAF mutations that occur in human cancer are a T1799A transversion mutation in exon 15, which results in a V600E amino acid substitution. V600E mutation accounts for 70 to 90% of BRAF mutant melanoma patients.

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