BRAF V600
mutation Detection Kit
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SKU
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100 Rxns : NBRQ22076
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50 Rxns : NBRQ22076_01
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20 Rxns : NBRQ22076_02
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BRAF V600 Mutation Detection Kit is an in-vitro multiplex, Real-Time PCR assay for the qualitative detection of V600 Mutations in the BRAF gene in human genomic DNA extracted from fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tumor tissue. The kit is for in-vitro diagnostic use and is intended to be used by trained professionals in a laboratory environment. The Kit differentiates the V600E mutation from the other V600 mutations like K/D/Ecomplex/M/R/G.
Features
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Single-Tube, Multiplex, TaqMan probe-based Qualitative Assay along with BRAF Wild
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RT-PCR Run Time is within 70 Minutes
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Open Platform Kit compatible with commercially available RT-PCR systems
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Traceability: HD238 BRAF V600E gDNA Reference Standard, 50%
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Sample Compatability: fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tumor tissue.
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Selective amplification of mutant-specific sequences in a mixture of wild-type and mutant DNA.
Storage & Precautions
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Recommended Storage: -20°C
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For Professional Use only.
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Read user manual
Clinical Relevance
BRAF mutations have been identified at a high frequency in specific cancers, including approximately 50 to 60% of melanoma. The cancers associated with the BRAF gene mutation are not specific to one part of the body or a certain cell type. These cancers include Melanoma, Hairy Cell Leukemia, non-Hodgkin lymphoma, Thyroid Cancer, Ovarian Cancer, Lung adenocarcinoma, Colorectal cancer, and Certain brain cancers including glioblastoma.
Approximately 90% of all identified BRAF mutations that occur in human cancer are a T1799A transversion mutation in exon 15, which results in a V600E amino acid substitution. V600E mutation accounts for 70 to 90% of BRAF mutant melanoma patients.